| Detailed information | |
| Title | Development and Validation of a High Sensitivity On-Line Extraction LC-MS/MS Method for the Determination of Exemestane and its Metabolite 17β-Hydroxyexemestane in Human Plasma |
| Summary | Exemestane is an orally active irreversible steroidal aromatiase inhibitor used for the therapy of metastatic postmenopausal breast cancer.The objective of this work was to develop and validate a highly sensitive on-line extraction LC-MS/MS method for the determination of exemestane (EXE) and 17β-hydroxy- exemestane (17β EXE) in human plasma.. A high sensitive, accurate and robust method for the determination of exemestane and its metabolite 17β-OH exemestane was developed. The study also demonstrates how the use of selective analyte isolation techniques, chromatography and MS/MS detection make it possible to analyse steroidal compounds susceptible to isobaric interferences at trace levels. |
| Category | 2. Symbiosis Application Notes These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated |
| Matrix | Plasma, Human Plasma |
| Market | Clinical, CRO |
| Technique | online SPE |
| Cartridge | Hysphere-C8 (10 x 2 mm) |
| Detection | AB SCIEX API 5000 |
| Compounds | exemestane (EXE) and 17β-hydroxy- exemestane (17β EXE) |
| Compound Group | exemestane (EXE) and 17β-hydroxy- exemestane (17β EXE) |
| Sample_volume | 100µL |
| Contact | Mohammed Abrar, John Allanson, David Browne, Jeremy Cook, Pratap Davuluri & Jennifer Dyer |
| Account | Unilabs York Bioanalytical Solutions, Cedar House, Northminster Business Park, Upper Poppleton, York YO26 6QR, UK |
| Published | Poster ASMS |
| Date | 04.08.2015 |
| Read PDF document | |
| Remarks | |
| Users | |
| Status | |