| Detailed information | |
| Title | Simultaneous Analysis of Seven Amphetamine Class Drugs in Urine for Forensic Toxicology |
| Summary | In this study, an assay has been developed for the simultaneous measurement of amphetamine class drugs from urine. T he method utilizes LC /MS and an online SPE system. T his combination of SPE sample preparation coupled with the analytical power of LC /MS is able to deliver an efficient assay for this commonly abused class of drug compounds. It was seen that urine contains a significant level of matrix interferences that cause ion suppression in the analysis of amphetamines. To optimize the sensitivity of methods for analyzing amphetamines using urine as a sample matrix, these interferences must be removed. T he use of the online SPE sample preparation in t he met hod was found to greatly reduce the matrix effects from urine, and allowed for measurement of amphetamines at significantly lower levels than simple urine “dilute and shoot” methods. T he reproducibility of sample analysis with online SPE was judged to be very good even when injecting samples onto different disposable online SPE cartridges. T he LC/MS system utilized in this work completely automated, and effectively integrated SPE sample preparation and LC/MS into a single platform. |
| Category | 2. Symbiosis Application Notes These are application notes that have been made in our application lab. They were based on customer demo's and customer method development requests. They include sorbent screening, a calibration curve and are optimized for carry over. They are not always (completely) validated |
| Matrix | Urine |
| Market | OSM Toxicology |
| Technique | online SPE |
| Cartridge | Waters ACQUIT Y Online SPE Manager C 18 Cartridge |
| Detection | Waters Xevo® TQD |
| Compounds | Amphetamine, Methamphetamine, Phentermine, ropylamphetamine, MDA, MDMA, MDEA |
| Compound Group | Amphetamine |
| Sample_volume | |
| Contact | Jeremy Netto, |
| Account | Waters Corporation |
| Published | Poster, Technical Brief |
| Date | 04.08.2015 |
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