Detailed information | |
Title | The measurement of plasma free metanephrines using automated on-line SPE HPLC-MS/MS |
Summary | The measurement of metanephrine and normetanephrine in raw human plasma with a minimal sample pre- treatment using spark Holland Symbiosis Pharma and Waters Quattro micro systems has been demonstrated. Considerable improvements in the sensitivity, selectivity and speed of the plasma free metanephrines assay have been provided when compared to previously published methods. The use of oasis WCX extraction cartridges has been shown to provide a selective sample clean up for highly basic analytes in a complex matrix such as human plasma. The combination of this selective clean up and Waters HILIC chemistry can provide a sensitive method of analysis for low level of highly polar, basic analytes. |
Category | 3. Symbiosis User Application These are applications that have been made by Symbiosis customers. They are validated and have been published. |
Matrix | Plasma |
Market | Clinical |
Technique | online SPE |
Cartridge | Oasis WCX |
Detection | MS (Waters Quattro Micro) |
Compounds | Metanephrine, normetanephrine |
Compound Group | Metanephrines |
Sample_volume | 20 µL |
Contact | Mr. Kendon Graham; Mr. Jan van der Molen, Mr. Ido Kema; Mr. Brian Keevil |
Account | Waters Chomatography; UMCG, Groningen, NL,: University of South Manchester, UK. |
Published | Final_PMets_720001943EN.pdf |
Date | 20.02.2008 |
Read PDF document | |
Remarks | Metanephrines (app note by Waters) |
Users | |
Status | Waters application note finished |