| Detailed information | |
| Title | The analysis of urinary free cortisol by on-line solid phase extraction LC/MS/MS |
| Summary | The measurement of urinary free Cortisol (UFC) is of clinical importance in the diagnosis of Cushing's syndrome. Cushing's is a disease caused by the autonomous and excessive production of cortisol. The majority of routine methods for determining 24 hr-UFC concentrations involve RIA or HPLC with UV detection. In general, the performance of immunoassays is limited due to the cross-reactivity of cortisol metabolites leading to the amounts of UFC being over estimated.1,2 In clinical laboratories, LC/MS/MS is now becoming more common due to the gains in specificity and accuracy compared to immunoassays.3In this application note, we describe the analysis of UFC by an automated on-line solid-phase extraction (XLC) MS/MS technique. The method uses a Spark Holland Symbiosis™ automated SPE instrument coupled to a Waters® Quattro micro™ tandem quadrupole mass spectrometer |
| Category | 3. Symbiosis User Application These are applications that have been made by Symbiosis customers. They are validated and have been published. |
| Matrix | Urine |
| Market | Clinical, Forensic |
| Technique | online SPE |
| Cartridge | Waters Oasis HLB |
| Detection | MS (Waters Quattro Micro) |
| Compounds | Cortisol |
| Compound Group | corticosteroids, glucocorticoid |
| Sample_volume | 20 µl |
| Contact | Mrs. Lisa J. Calton, Mr. Gareth Hammond, Mr. Brian Keevil |
| Account | Waters Corporation, Manchester and South Manchester University Hospital, Manchester, UK |
| Published | Poster AACC 2007 |
| Date | 20.02.2008 |
| Read PDF document | |
| Remarks | Limit of quantification is 0.5 ng/mL with a sample injection volume of 20 µL |
| Users | |
| Status | Finished |