| Detailed information | |
| Title | Method Development and Validation for Dried Blood Spot Analysis based on Flow Through Desorption, Solid-Phase Extraction and Mass Spectrometry |
| Summary | A new SPE-LC-MS/MS method for analysis of very small blood volumes from DBS cards was developed and validated. 1 μL-blood spots are desorbed in a flow through manner directly onto a SPE-LC-MS/MS system cartridge for online clean-up, followed by LC/MS/MS analysis. |
| Category | 1. Prospekt 1 Application information These applications were mostly done on Prospekt1 and can offer a good starting point when executed on Symbiosis. Care should be taken on differences in volume (SDU vs. HPD). Also, most information is based on other detectors than MS |
| Matrix | mouse blood |
| Market | Clinical |
| Technique | DBS |
| Cartridge | HySphere C18HD 10x2 mm, 7 μm |
| Detection | APCI-MS (4000 QTrap, AB Sciex) |
| Compounds | Phospholipids |
| Compound Group | pharmacokinetic studies |
| Sample_volume | 1 µL |
| Contact | Chris Swaanen, Benno Ingelse and Christel Hempen |
| Account | Spark Holland, P. de Keyserstraat 8, PO Box 388, 7800AJ, Emmen, The Netherlands |
| Published | poster |
| Date | 02.12.2019 |
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| Remarks | |
| Users | |
| Status | poster |