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Title A Quantitative LC-MS Method to Determine Lutein Levels in Whole Human Blood Using Dried Blood Spot Analysis (DBS)
Summary It is believed that lutein keeps eyes safe from oxidative stress and the highenergy photons of blue light. Further, studies have shown that an increase in macula pigmentation, such as lutein, may decrease the risk for disease such as macular degeneration (AMD). There’s also evidence to suggest that consumption of lutein and zeaxanthin lowers the risk of cataract development. An additional benefit of consuming lutein and zeaxanthin is the improvement of visual performance and decrease in light sensitivity (glare). In the work presented here, we have used dried blood spot (DBS) technology in conjunction with addition of an anti-oxidant that is prespotted on the DBS paper in order to stabilize the analyte and thereby preventing oxidation and degradation. Using this approach has led to reliable sensitivity and quantitation down to 10 ppb.
Category 1. Prospekt 1 Application information

These applications were mostly done on Prospekt1 and can offer a good starting point when executed on Symbiosis. Care should be taken on differences in volume (SDU vs. HPD). Also, most information is based on other detectors than MS
 
Matrix Blood
Market Clinical
Technique DBS
Cartridge
Detection MSWaters Xevo® TQ-S
Compounds Lutein
Compound Group Xanthophyll, Carotinoid, Food coloring, anti-oxidants
Sample_volume 20 µL
 
Contact Todime M Reddy, Stefan Ehling, Carl Norén
Account AR&S, Columbus, OH, USA
Published poster
Date 19.11.2019
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Status poster